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oligo dt 20  (Jena Bioscience)


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    Structured Review

    Jena Bioscience oligo dt 20
    Oligo Dt 20, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligo+dt+20/Oligo+(dT)20/bio_rxiv__64898__2026__03__27__714762-239-28-31
    Average 94 stars, based on 4 article reviews
    oligo dt 20 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Plasticity in nonsense-mediated decay and translation initiation regulate polyphenism
    Article Snippet: DNase treatment and RNA purification were performed with the RNA Clean and Concentrator Kit-25 (Zymo Research Cat. No. R1018). .. 1 μg of RNA was reverse transcribed using the manufacturer protocol of SuperScript III Reverse Transcriptase (Invitrogen Cat. No. 18080044) in a reaction containing additional 2.5 μM of oligo (dT) 20 (Jena Bioscience Cat. No. PM-304), 0.5 mM dNTPs, 40 U RNaseOUT recombinant ribonuclease inhibitor (Invitrogen Cat. No. 10777019), and 200 U of the reverse transcriptase. .. The qPCR reactions were performed with Luna Universal qPCR Master Mix (New England Biolabs Cat. No. M3003), with 0.25 μM of each primer and 1 and 2 μl of the cDNA for sult-8 and eud-1 reactions, respectively.

    Article Title: Effect of a Bone Morphogenetic Protein-2-derived peptide on the expression of tumor marker ZNF217 in osteoblasts and MCF-7 cells
    Article Snippet: The cell proliferation assay was conducted with a BrdU cell proliferation kit (MilliporeSigma, USA). .. The following reagents were used in gene expression analysis: NucleoSpin RNA Kit (MACHEREY-NAGEL, Germany), M-MuLV Reverse Transcriptase (MINOTECH Biotechnology, Greece), oligo(dT) 20 (Jena Bioscience, Germany), dNTP mix (MINOTECH biotechnology, Greece), Dithiothreitol (DTT) solution (MINOTECH biotechnology, Greece) and KAPA SYBR FAST qPCR Kit Master Mix (2×) ABI Prism (MilliporeSigma, Germany). .. Disposable supplies were purchased from Thermo Fisher Scientific (USA), KISKER BIOTECH (Germany) and SARSTEDT (Germany).

    Recombinant:

    Article Title: Plasticity in nonsense-mediated decay and translation initiation regulate polyphenism
    Article Snippet: DNase treatment and RNA purification were performed with the RNA Clean and Concentrator Kit-25 (Zymo Research Cat. No. R1018). .. 1 μg of RNA was reverse transcribed using the manufacturer protocol of SuperScript III Reverse Transcriptase (Invitrogen Cat. No. 18080044) in a reaction containing additional 2.5 μM of oligo (dT) 20 (Jena Bioscience Cat. No. PM-304), 0.5 mM dNTPs, 40 U RNaseOUT recombinant ribonuclease inhibitor (Invitrogen Cat. No. 10777019), and 200 U of the reverse transcriptase. .. The qPCR reactions were performed with Luna Universal qPCR Master Mix (New England Biolabs Cat. No. M3003), with 0.25 μM of each primer and 1 and 2 μl of the cDNA for sult-8 and eud-1 reactions, respectively.

    Gene Expression:

    Article Title: Effect of a Bone Morphogenetic Protein-2-derived peptide on the expression of tumor marker ZNF217 in osteoblasts and MCF-7 cells
    Article Snippet: The cell proliferation assay was conducted with a BrdU cell proliferation kit (MilliporeSigma, USA). .. The following reagents were used in gene expression analysis: NucleoSpin RNA Kit (MACHEREY-NAGEL, Germany), M-MuLV Reverse Transcriptase (MINOTECH Biotechnology, Greece), oligo(dT) 20 (Jena Bioscience, Germany), dNTP mix (MINOTECH biotechnology, Greece), Dithiothreitol (DTT) solution (MINOTECH biotechnology, Greece) and KAPA SYBR FAST qPCR Kit Master Mix (2×) ABI Prism (MilliporeSigma, Germany). .. Disposable supplies were purchased from Thermo Fisher Scientific (USA), KISKER BIOTECH (Germany) and SARSTEDT (Germany).

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of a Bone Morphogenetic Protein-2-derived peptide on the expression of tumor marker ZNF217 in osteoblasts and MCF-7 cells
    Article Snippet: The cell proliferation assay was conducted with a BrdU cell proliferation kit (MilliporeSigma, USA). .. The following reagents were used in gene expression analysis: NucleoSpin RNA Kit (MACHEREY-NAGEL, Germany), M-MuLV Reverse Transcriptase (MINOTECH Biotechnology, Greece), oligo(dT) 20 (Jena Bioscience, Germany), dNTP mix (MINOTECH biotechnology, Greece), Dithiothreitol (DTT) solution (MINOTECH biotechnology, Greece) and KAPA SYBR FAST qPCR Kit Master Mix (2×) ABI Prism (MilliporeSigma, Germany). .. Disposable supplies were purchased from Thermo Fisher Scientific (USA), KISKER BIOTECH (Germany) and SARSTEDT (Germany).



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